Iron Metabolism-related Gene Editing Services

jakemiller — Tue, 27 Nov 2018 14:07:49 +0000

Iron is essential for life, but it can also lead to cell death. As a result, cells have evolved a robust and tightly regulated set of genes to maintain iron homeostasis.
Iron Metabolism Gene Mutations
Iron in the body is in a dynamic balance of constant absorption, utilization, storage and circulation. The maintenance of iron homeostasis is dependent on the complex and delicate regulation of the body, and its maintenance depends on the proper functioning of iron regulation mechanisms at all levels of the body.

Regulation of Hepcidin SynthesisRegulation of hepcidin expression [1].
Systemic Iron Homeostasis
The maintenance of systemic iron homeostasis is dependent on a small molecule protein secreted by hepatocytes, hepcidin, which binds to FPN on the surface of target cell membranes leading to internalisation and degradation of FPN, thereby reducing iron export into the circulation.

Regulation of Hepcidin Synthesis
TFR2: TFR2 is predominantly expressed in the liver and mice and humans with TFR2 mutations have severe haemochromatosis and very low levels of hepcidin, suggesting its important role in hepcidin regulation.

HFE: Mutations in the HFE gene, which is localised on human chromosome 1q21, can cause severe haemochromatosis and reduced hepcidin levels in HFE knockout mice. It was found that HFE may act by binding to TFR2 to form a complex.

Stem Cell Therapy Development Service for T1DM

jakemiller — Tue, 27 Nov 2018 14:05:19 +0000

Stem Cells Therapy in The Treatment of T1DM
Stem cells open up a wide spectrum of diabetes research possibilities. Stem cell therapy using islet-like insulin-producing cells derived from human pluripotent stem cells has the potential to allow patients with T1DM to withdraw from insulin therapy. Furthermore, autologous transplantation using induced pluripotent stem cells-derived islet cells is expected to have the potential to treat T1DM patients without requiring immunosuppressive therapy.

Services

Stem Cell Reprogramming

Sendai virus (RNA virus) based reprogramming can be used to reprogram a wide range of cell types and origins. The reprogramming process is based on transfection of transcriptional factor genes (Oct4, Sox2, c-Myc and Klf4).

Stem Cell Culture

We offer a serum-free medium for induced pluripotent stem cells that maintain pluripotency, trilineage differentiation potential and karyotypic stability.

Stem Cell Engineering

Using lipid-base transfection, electroporation, viral approaches or genome engineering tools such as CRISPR or TALEN.

Stem Cell Differentiation

Using consecutive cytokines or signaling modulators to control the differentiation of iPSCs into pancreatic β-cells. Identifying the proliferative signals as well as instructive signals that induce the differentiation process.

Stem Cell Characterization

Includes assessing pluripotency, genomic stability, and safety. And the safety assays include sterility, mycoplasma, endotoxin, and adventitious viral testing.

Julia Community 🟣: jakemiller

Iron Metabolism-related Gene Editing Services

Stem Cell Therapy Development Service for T1DM